The detection of autoantibodies with an enzyme bridge method.
نویسندگان
چکیده
The horseradish peroxidase bridge method described by Mason, Phifer, Spicer, Swallow, and Dreskin (1969) has been found to be as sensitive and specific as fluorescence methods for detecting antinuclear factor and gastric parietal cell antibody, and to a lesser extent, antibodies to smooth muscle and mitochondria. However, the method has at least four practical advantages over fluorescence techniques.
منابع مشابه
Development of an ELISA Based Method for the Detection of Pemphigus vulgaris Autoantibodies
For the evaluation of autoantibodies in Pemphigus vulgaris (PV), an indirect ELISA assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (M.W.) of 130-160 kDa. The evaluation of Pemphigus IgG, IgM and IgA autoantibodies in 75 patients and 50 control sera indicated that this indirect ELISA assay was a useful method for the detection of ...
متن کاملDamage Detection of Bridge by Rayleigh-Ritz Method
As a result of environmental and accidental actions, damage occurs in structures. The early detection of any defect can be achieved by regular inspection and condition assessment. In this way, the safety and reliability of structures can be increased. This paper is devoted to propose a new and effective method for detecting, locating, and quantifying beam-like structures. This method is based o...
متن کاملAUTOANTIBODIES AGAINST THYR OID CONSTITUENTS AND CIC IN GRAVES\' DISEASE ASSOCIATED WITH EXOPH THALMUS
32 patients (19 female. 13 male)• suffering from Graves' disease with exophthalmus and five without exophthalmus were studied for various autoantibodies including anti-thyroglobulin (ATAB). anti-microsomal antibody (AMAB). anti-parietal antibodies (APAB) and also circulating immune complex (CrC). 43 normal subjects were tested concurrently. Immunofluorescent technique was employed for auto...
متن کاملDamage detection of multi-girder bridge superstructure based on the modal strain approaches
The research described in this paper focuses on the application of modal strain techniques on a multi-girder bridge superstructure with the objectives of identifying the presence of damage and detecting false damage diagnosis for such structures. The case study is a one-third scale model of a slab-on-girder composite bridge superstructure, comprised of a steel-free concrete deck with FRP rebars...
متن کاملDAMAGE DETECTION OF BRIDGE STRUCTURES IN TIME DOMAIN VIA ENHANCED COLLIDING BODIES OPTIMIZATION
In this paper, a method is presented for damage detection of bridges using the Enhanced Colliding Bodies Optimization (ECBO) utilizing time-domain responses. The finite element modeling of the structure is based on the equation of motion under the moving load, and the flexural stiffness of the structure is determined by the acceleration responses obtained via sensors placed in d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical pathology
دوره 27 2 شماره
صفحات -
تاریخ انتشار 1974